Cu(I) recognition via cation-
and methionine interactions in CusF
Methionine-rich motifs have an important role in copper trafficking factors, including the CusF protein. Here we show that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies demonstrate that both thioether ligation and strong cation-
interactions with tryptophan stabilize metal binding. This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry.Yi Xue, Anna V Davis, Gurusamy Balakrishnan, Jay P Stasser, Benjamin M Staehlin, Pamela Focia, Thomas G Spiro, James E Penner-Hahn and Thomas V O'Halloran
No comments:
Post a Comment